Until the end of February, day-trippers will be asked to park at a tourism centre 2 kilometres away and pay for a shuttle bus.
The official website for Ginzan Onsen said the trial had been launched after years of debate, adding that authorities were hoping to maintain a good relationship with visitors.
When heavy traffic recently stopped an ambulance, “an emergency service team made it to the scene by running, but it was truly a life-and-death situation”, the site said.
“Some visitors are verbally abusive” when officials come to help cars stuck in snow, according to the site, which it described as “unbearable”.
More than 33 million foreign visitors have entered Japan so far this year, breaking 2019’s full-year record of nearly 32 million.
Locals in Japan’s ancient capital Kyoto have complained of tourists harassing the city’s famed geisha and decided to ban visitors from entering private alleys.
Authorities have also introduced an entry fee and daily cap on numbers on Mount Fuji’s most popular trail, while barriers were installed outside a convenience store nearby to stop crowds of snap-happy visitors getting injured on the road.